Application Notes: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 28.7 kDa. The protein migrates as 40-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation
Biotinylated Human TNFR2, His, Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human TNF-alpha, His Tag (active trimer) (MALS verified) at 5 ug/ml (100 uL/well) can bind Biotinylated Human TNFR2, His, Avitag with a linear range of 0.2-5 ng/mL.
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