Application Notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 62.0 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation
Human LGR5, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human R-Spondin 3 at 2 ug/ml (100 uL/well) can bind Human LGR5, His Tag with a linear range of 5-39 ng/mL.
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