20mM PB (pH 7.4). 5 % trehalose is added as protectant before lyophilization.
Purity:
> 95% as determined by Bis-Tris PAGE, > 95% as determined by HPLCEndotoxin level is less than 1EU per ug by the LAL method.
Form:
Lyophilized
Sequence:
Arg319 - Phe541 (N501Y)
Application Notes:
Application Notes: SDS-PAGE
Immobilized SARS-CoV S protein RBD (N501Y), His Tag at 0.5 ug/ml (100ug/Well) on the plate. Dose response curve for Human ACE2, Fc Tag with the EC50 of 12.7 ng/ml determined by ELISA.
The purity of SARS-COV-2 S protein RBD (N501Y) is greater than 95% as determiend by SEC-HPLC.
Human ACE2 captured in protein A chip, can bind SARS-CoV S protein RBD (N501Y), His Tag with an affinity constant of 1.74nM as determined in SPR assap (Biacore T200).
SARS-COV2 S protein RBD (N501Y) on Tris-Bis PAGE under reduced condition. The purity os great than 95%.
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