Binding Assay of ACE2 and SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein. Coating: SARS-CoV-2 (COVID-19) Spike S1 recombinant protein (orb1231589) from 3 ng to 1000 ng. Capture: ACE2 recombinant protein, orb1231604 (0.5 µg/mL). Secondary: Goat anti-human IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation with SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) Spike S1 recombinant protein, orb1231589. Antibodies: SARS-CoV-2 (COVID-19) Spike S1, orb1239981, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 µg/mL and Lane 2: 2 µg/mL.
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