Anti-PEX10 antibody (orb1238073) was raised against a peptide corresponding to 10 amino acids near the carboxyl terminus of human PEX10. The immunogen is located within last 50 amino acids of PEX10.
Conjugation:
Unconjugated
Alternative Names:
PEX10 Antibody: Peroxin-10, Peroxisomal biogenesis factor 10, Peroxisome assembly protein 10, RING finger protein 69, RNF69
PEX10 Antibody is supplied in PBS containing 0.02% sodium azide.
Form:
Liquid
Target:
PEX10
WB Validation in Human and Mouse Cell Lines. Loading: 15 µg of lysate. Antibodies: PEX10 orb1238073, 4 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
WB Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: PEX10 orb1238073, 4 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
WB Validation in Rat Tissues. Loading: 15 µg of lysates per lane. Antibodies: PEX10 orb1238073, 4 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunofluorescence Validation of PEX10 in Human Daudi Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling PEX10 with orb1238073 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunofluorescence Validation of PEX10 in Human Spleen. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling PEX10 with orb1238073 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunofluorescence Validation of PEX10 in Mouse Thymus. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus labeling PEX10 with orb1238073 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunofluorescence Validation of PEX10 in Rat Testis. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PEX10 with orb1238073 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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