Anti-AIFM2 antibody ( orb1238078) was raised against a peptide corresponding to 12 amino acids near the carboxyl terminus of human AIFM2. The immunogen is located within the last 50 amino acids of AIFM2.
AIFM2 Antibody is supplied in PBS containing 0.02% sodium azide.
Form:
Liquid
Target:
AIMF2
WB Validation in Human Mouse and Rat Cell Lines. Loading: 15 µg of lysate. Antibodies: AIFM2 orb1238078, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10000 dilution.
WB Validation in Mouse Tissues. Loading: 15 µg of lysate. Antibodies: AIFM2 orb1238078, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10000 dilution.
WB Validation in Rat Tissues. Loading: 15 µg of lysate. Antibodies: AIFM2 orb1238078, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10000 dilution.
Immunofluorescence Validation of AIFM2 in HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling AIFM2 with orb1238078 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunohistochemistry Validation of AIFM2 in Human Testis. Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-AIFM2 antibody (orb1238078) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of AIFM2 in Mouse Spleen. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-AIFM2 antibody (orb1238078) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of AIFM2 in Rat Testis. Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-AIFM2 antibody (orb1238078) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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