Avian influenza neuraminidase antibody was raised against a synthetic peptide corresponding to 15 amino acids near the carboxy terminus of the avian influenza neuraminidase protein.Efforts were made to use relatively conserved regions as the antigen.The immunogen is located within the last 50 amino acids of Avian Influenza Neuraminidase.
Avian Influenza Neuraminidase Antibody is supplied in PBS containing 0.02% sodium azide.
Form:
Liquid
Target:
NA
Application Notes:
Application Notes: WB: 1 µg/mL. Avian Influenza Neuraminidase antibody can be used for the detection of the Neuraminidase protein from the H5N1 strain of avian influenza A in ELISA and WB. It will detect 10 ng of free peptide at 1 µg/mL. All other applications and species not yet tested
Western Blot Validation with Avian Influenza NA Protein. Loading: 50 ng (Lane 1) and 100 ng (Lane 2) Avian Influenza Neuraminidase recombinant protein. Antibodies: Avian Influenza antibody, orb1238121, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
ELISA Validation with Avian Influenza NA Protein. Coating Antigen: Avian Influenza Neuraminidase recombinant protein, 2 µg/mL, incubated at 4C overnight. Detection Antibodies: Avian Influenza NA antibody, orb1238121, dilution: 1-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:10000, incubated at RT for 1 hr.
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