Anti-SARS-CoV-2 (COVID-19) ORF7a antibody (orb1239059) was raised against a peptide corresponding to 15 amino acids near the central domain of SARS-CoV-2 (COVID-19) ORF7a protein. The immunogen is located within 40-90 amino acids of the SARS-CoV-2 (COVID-19) ORF7a protein.
Conjugation:
Unconjugated
Alternative Names:
Accessory protein 7a, ORF7a, ORF7a protein, Protein U122, Protein X4, covid-19, sars-cov-2
SARS-CoV-2 (COVID-19) ORF7a antibody is supplied in PBS containing 0.02% sodium azide.
Form:
Liquid
Target:
ORF7a protein, Accessory protein 7a, Protein U122, Protein X4, ORF7a
Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) ORF7a in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) ORF7a antibody (orb1239059, 1 µg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 ORF7a protein was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.
ELISA Validation. Antibodies: SARS-CoV-2 (COVID-19) ORF7a Antibody, orb1239059. A direct ELISA was performed using SARS-CoV-2 ORF7a immunogen peptide (orb1231669) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF7a antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL.
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