Anti-SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody (orb1239090) was raised against a peptide corresponding to 15 amino acids in the center of SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) protein. The immunogen is located within 80-130 amino acids of the SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) protein.
Conjugation:
Unconjugated
Alternative Names:
3C-like proteinase, 3CLp, nsp5, Non-structure protein 5, Main protease, Mpro
SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody is supplied in PBS containing 0.02% sodium azide.
Form:
Liquid
Target:
3CL-PRO
ELISA Validation with SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Protein. Antibodies: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody, orb1239090. A direct ELISA was performed using SARS-CoV-2 3CL-PRO (NSP5) recombinant protein (orb1231584) as coating antigen and the anti-SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL.
Western Blot Validation with SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) recombinant protein (orb1231584). Antibodies: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5), orb1239090, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 µg/mL and. Lane 2: 1 µg/mL.
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