ATF6 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human ATF6.The immunogen is located within amino acids 30 - 80 of ATF6.
ATF6 Antibody is supplied in PBS containing 0.02% sodium azide.
Form:
Liquid
Target:
ATF6
Application Notes:
Application Notes: WB: 0.5-2 µg/mL, IHC: 5 µg/mLAntibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested
KO Validation of ATF6, Loading: 15 µg of human HeLa cell lysate Antibodies ATF6 orb1239224, 0.5 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
WB Validation in Human Cell Lines, Loading: 15 µg of human cell lysate Antibodies ATF6 orb1239224, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. conjugate at 1:10000 dilution. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. conjugate at 1:10000 dilution.
WB Validation in Mouse Tissue, Loading: 15 µg of mouse tissue lysate Antibodies: ATF6 orb1239224, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
WB Validation in Rat Heart, Loading: 15 µg of rat kidney lysate Antibodies: ATF6 orb1239224, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry Validation of ATF6 in Human Testis Tissue, Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-ATF6 antibody (orb1239224) at 5 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of ATF6 in Mouse Kidney Tissue, Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ATF6 antibody (orb1239224) at 5 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of ATF6 in Rat Kidney Tissue, Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ATF6 antibody (orb1239224) at 5 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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