Caspase-8 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Caspase-8 isoform A.The immunogen is located within the last 50 amino acids of Caspase-8.
Caspase-8 Antibody is supplied in PBS containing 0.02% sodium azide.
Form:
Liquid
Target:
CASP8
Application Notes:
Application Notes: Caspase-8 antibody can be used for the detection of caspase-8 by Western blot at 0.5 to 2 µg/mL. Antibody can also be used for immunocytochemistry starting at 2 µg/mL and immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.Antibody validated: Western Blot in human, mouse, and rat samples, Immunohistochemistry in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested
Immunocytochemistry of caspase-8 in Jurkat cells with caspase-8 antibody at 2 µg/mL.
Immunohistochemistry of Caspase-8 in human spleen tissue with Caspase-8 antibody at 5 µg/mL.
Immunofluorescence of Caspase-8 in human spleen tissue with Caspase-8 antibody at 20 µg/mL. Red: Caspase-8 Antibody (orb1239264). Blue: DAPI staining.
Immunofluorescence of Caspase-8 in Jurkat cells with Caspase-8 antibody at 20 µg/mL.
Immunohistochemistry of Caspase-8 in human spleen tissue with Caspase-8 antibody at 5 µg/mL.
WB Validation in Human K562 Cells. Loading: 15 µg of K562 cell lysate Antibodies: Casp8 orb1239264, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
WB Validation in Mouse 3T3/NIH Cells. Loading: 15 µg of 3T3/NIH cell lysate Antibodies: Casp8 orb1239264, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
WB Validation in Rat YB2/0 Cells. Loading: 15 µg of YB2/0 cell lysate Antibodies: Casp8 orb1239264, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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