Anti-IFN-beta antibody (orb1239454) was raised against a peptide corresponding to 17 amino acids near the center of human IFN-beta. The immunogen is located within amino acids 110-160 of IFN-beta.
IFN-beta Antibody is supplied in PBS containing 0.02% sodium azide.
Form:
Liquid
Target:
IFNB1
Western Blot Validation in Mouse A20 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: IFN-beta orb1239454 (Lane 1: 5 µg/mL and Lane 2: 10 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Independent Antibody Validation (IAV) via Protein Expression Profile in Human and Mouse Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: IFN-beta orb1239454 (4 µg/mL), IFN-beta, (5 µg/mL) and beta-actin orb1240312 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Multiple Tissues and Recombinant Protein. Loading: 15 µg of lysates per lane. Antibodies: IFN-beta orb1239454 (4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry Validation of IFN-beta in Human Liver Tissue. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IFN-beta antibody (orb1239454) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunofluorescence Validation of IFN-beta in Human Liver Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed human liver cells labeling IFN-beta with orb1239454 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Immunohistochemistry Validation of IFN-beta in Human Liver Tissue. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IFN-beta antibody (orb1239454) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunofluorescence Validation of IFN-beta in Mouse Liver Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse liver tissue labeling IFN-beta with orb1239454 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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