IRF7 Antibody, Unconjugated, Rabbit, Polyclonal

Article Name:	IRF7 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	BYT-ORB1239524
Supplier Catalog Number:	orb1239524
Alternative Catalog Number:	BYT-ORB1239524-0.02,BYT-ORB1239524-0.1
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IHC-P, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Anti-IRF7 antibody (orb1239524) was raised against a peptide corresponding to 17 amino acids near the center of human IRF7. The immunogen is located within amino acids 200-230 of IRF7.
Conjugation:	Unconjugated
Alternative Names:	IRF7 Antibody: IRF7A, IRF7B, IRF7C, IRF7H, IRF-7H, Interferon regulatory factor 7, IRF-7

IRF7 Antibody

Clonality:	Polyclonal
Concentration:	1 mg/mL
NCBI:	92985
UniProt:	Q92985
Buffer:	IRF7 Antibody is supplied in PBS containing 0.02% sodium azide.
Form:	Liquid
Target:	IRF7

	KO Validation in A549 Cell Lysate. Loading: 10 µg of A549 WT cell lysates or IRF7 KO cell lysates. Antibodies: IRF7 orb1239524 (0.5 µg/mL) and beta-actin orb1240312 (1 µg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
	Immunohistochemistry Validation of IRF7 in Mouse Pancreas Tissue. Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-IRF7 antibody (orb1239524) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Immunohistochemistry Validation of IRF7 in Rat Brain Tissue. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-IRF7 antibody (orb1239524) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: IRF7, orb1239524, (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Western Blot Validation in Human Tissues. Loading: 15 µg of lysates per lane. Antibodies: IRF7, orb1239524 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: IRF7, orb1239524 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Western Blot Validation in Rat Tissues. Loading: 15 µg of lysates per lane. Antibodies: IRF7, orb1239524 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Immunofluorescence Validation of IRF7 in Mouse Raw264.7 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Raw264.7 cells labeling IRF7 with orb1239524 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
	Immunohistochemistry Validation of IRF7 in Human Colon Tissue. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-IRF7 antibody (orb1239524) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Immunohistochemistry Validation of IRF7 in Human Pancreas Tissue. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-IRF7 antibody (orb1239524) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Immunohistochemistry Validation of IRF7 in Human Liver Tissue. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IRF7 antibody (orb1239524) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.