LAG-3 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Form:
Liquid
Target:
LAG3
Application Notes:
Application Notes: LAG-3 antibody can be used for ELISA starting at 0.25 µg/mL. For Immunocytochemistry start at 1 µg/mL. For Immunofluorescence start at 10 µg/mL. For Immunohistochemistry start at 5 µg/mL. For Flow Cytometry start at 1 µg/ml.Antibody validated: Immunohistochemistry in human samples, Immunocytochemistry in human samples, Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested
Immunocytochemistry of LAG-3 in over expressing HEK293 cells using LAG-3 antibody and control mouse IgG antibody (left corner box) at 1 µg/ml.
Immunofluorescence of LAG-3 in over expressing HEK293 cells using LAG-3 Antibody at 2 µg/ml. Green: LAG3 Antibody [9F9] (orb1239551) Blue: DAPI staining
Immunofluorescence of LAG-3 in over human spleen tissue using LAG-3 Antibody at 10 µg/ml. Red: LAG3 Antibody [9F9] (orb1239551) Blue: DAPI staining
Immunohistochemistry of LAG-3 in human lymphoma tissue using LAG-3 Antibody and control mouse IgG (corner box) at 5 µg/ml.
Flow cytometry analysis of LAG-3 over expressing HEK293 cells using LAG-3 antibody at 1 µg/ml. Blue: untransfected HEK293 cells. Yellow: LAG-3 over expressing HEK293 cells.
Titration curve analysis of LAG-3 mAbs to detect recombinant LAG-3 in ELISA with orb1239547, orb1239545, orb1239551, orb1239549, orb1239550, orb1239548, and orb1239546 antibodies at decreasing concentrations.
A sandwich ELISA was performed using the anti-LAG3 mAbs as the capture antibodies for the LAG3 extracellular domain antigen with biotin-labeled Risk-Free anti-LAG3 mAbs as the detection antibodies.
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