Anti-PGAM5 antibody (orb1239864) was raised against a peptide corresponding to 14 amino acids near the carboxyl terminus of human PGAM5. The immunogen is located within the last 50 amino acids of PGAM5.
Conjugation:
Unconjugated
Alternative Names:
Serine/threonine-protein phosphatase PGAM5 mitochondrial, Bcl-XL-binding protein v68, Phosphoglycerate mutase family member 5
PGAM5 Antibody is supplied in PBS containing 0.02% sodium azide
Form:
Liquid
Target:
PGAM5
Western Blot Validation in Mouse and Rat Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: PGAM5, orb1239864 0.5 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: PGAM5, orb1239864 0.5 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: PGAM5, orb1239864 0.5 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Rat Tissues. Loading: 15 µg of lysates per lane. Antibodies: PGAM5, orb1239864 0.5 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunofluorescence Validation of PGAM5 in Human Liver. Immunofluorescent analysis of 4% paraformaldehyde-fixed human liver labeling PGAM5 with orb1239864 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunofluorescence Validation of PGAM5 in Mouse Testis. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse testis labeling PGAM5 with orb1239864 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunofluorescence Validation of PGAM5 in Rat Testis. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PGAM5 with orb1239864 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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