Anti-SPT2 antibody (orb1240010) was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human SPT2. The immunogen is located within the last 50 amino acids of SPT2.
Conjugation:
Unconjugated
Alternative Names:
SPT2 Antibody: LCB2, SPT2, HSN1C, LCB2A, NSAN1C, hLCB2a, KIAA0526, LCB2, Serine palmitoyltransferase 2, Long chain base biosynthesis protein 2, LCB 2
SPT2 Antibody is supplied in PBS containing 0.02% sodium azide.
Form:
Liquid
Target:
SPTLC2
Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: SPT2, orb1240010 (1 µg/mL), SPT2 (1 µg/mL), and beta-actin (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: SPT2, orb1240010 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse 3T3 Cell Lysate in (A) the absence and (B) the presence of blocking peptide. Loading: 15 µg of lysates per lane. Antibodies: SPT2, orb1240010 (0.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Human Brain Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: SPT2, orb1240010 (Lane 1: 0.5 µg/mL and Lane 2: 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse 3T3/NIH Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: SPT2, orb1240010 (Lane 1: 0.5 µg/mL and Lane 2: 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunocytochemistry Validation of SPT2 in Mouse 3T3 Cells. Immunocytochemical analysis of 3T3 cells using anti-SPT2 antibody (orb1240010) at 10 µg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunofluorescence Validation of SPT2 in Mouse 3T3 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed 3T3 Cells labeling SPT2 with orb1240010 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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