Anti-STAT1 alpha antibody (orb1240037) was raised against a peptide corresponding to 38 amino acids near the carboxy terminus of human STAT1 alpha. The immunogen is located within the last 50 amino acids of STAT1 alpha.
STAT1 alpha Antibody is supplied in PBS containing 0.02% sodium azide.
Form:
Liquid
Target:
STAT1
Western Blot Validation in Human and Mouse Cell Lines of (H) Hela, (J) Jurkat, (A) A431, (K) K562 and (N) 3T3/NIH. Loading: 15 µg of lysates per lane. Antibodies: STAT1 alpha orb1240037 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: STAT1 alpha orb1240037 (1 µg/mL), competitor antibody (2 µg/mL), and beta-actin (1.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse tissues. Loading: 15 µg of lysates per lane. Antibodies: STAT1 alpha orb1240037 (0.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Rat Cell Line. Loading: 15 µg of lysates per lane. Antibodies: STAT1 alpha orb1240037 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunofluorescence Validation of STAT1 alpha in Human Colon Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Colon Tissue labeling STAT1 alpha with orb1240037 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunohistochemistry Validation of STAT1 alpha in Human Colon Tissue. Immunohistochemical analysis of paraffin-embedded Human Colon Tissue using anti-STAT1 alpha antibody (orb1240037) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunocytochemistry Validation of STAT1 alpha in Human HeLa Cells. Immunocytochemical analysis of HeLa cells using anti-STAT1 alpha antibody (orb1240037) at 10 µg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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