Anti-ACE2 antibody (biotin) was raised against a peptide corresponding to 18 amino acids near the carboxy terminus of human ACE2. The immunogen is located within the last 50 amino acids of ACE2.
ACE2 Antibody is supplied in PBS containing 0.02% sodium azide.
Form:
Liquid
Target:
ACE2
Application Notes:
Application Notes: IHC: 2 µg/mL Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested
ELISA Validation. Coating Antigen: immunogen peptide, orb1236704, 10 ug/mL, incubate at 4 C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, orb1240301 or orb1239144, dilution: 0.5-1000 ng/mL, incubate at RT for 1 hr. orb1240301 was detected by HRP-conjugated streptavidin at 1:5000 and orb1239144 was detected by anti-rabbit HRP conjugated secondary antibodies at 1:10000, incubate at RT for 1 hr.
Immunohistochemistry Validation of ACE-2 in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded human testis tissue using anti- ACE2 antibody (orb1240301, 5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C, following by streptavidin-HRP conjugate at 20 ug/mL. Counter stained with Hematoxylin.
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