Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Form:
Liquid
Target:
ERG
Application Notes:
Application Notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 55kDa band observed in lysates of cell line MOLT4 (calculated MW of 53.8kDa according to NP_891548.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour
orb1247546 (0.1 ug/ml) staining of MOLT4 lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1247546 (10 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
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