The immunogen for this antibody is: C-HRRSMPVDERDLQ
Conjugation:
Unconjugated
Alternative Names:
RRAD, Ras-related associated with diabetes, RAD, RAD1, REM3, GTP-binding protein RAD, OTTHUMP00000174805, RAS (RAD and GEM) like GTP binding 3, ras associated with diabetes
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Form:
Liquid
Target:
RRAD
Application Notes:
Application Notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 37kDa band observed in Mouse Heart lysates and no 37kDa in the KO lysates (calculated MW of 33.2kDa according to mouse NP_062636.2 and human NP_004156.1). There is some non-specific background, we call for caution when used for other assays than Western blot. In transfected HEK293 transiently expressing Mouse Rrad (HA tagged), a band of approx. 45kDa is observed. No bands are observed in transfected HEK293 transiently expressing related Mouse GTPases. Recommended concentration: 0.5-2µg/mL
A): orb1248716 (0.5 ug/ml) staining of HEK293 lysates overexpressing several HA-tagged Mouse GTPases, including Rrad (10 ug protein in RIPA buffer) and compared with an HA-specific antibody. B): orb1248716 (0.5 ug/ml) staining of WT and KO lysates of Mouse Heart.
* VAT and and shipping costs not included. Errors and price changes excepted
