Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Form:
Liquid
Target:
UBE2C
Application Notes:
Application Notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 19kDa band observed in lysates of cell lines HEK293 and HeLa (calculated MW of 19.7kDa according to NP_008950.1). Recommended concentration: 1-3µg/mL
orb1248769 (1 ug/ml) staining of HEK293 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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