Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Form:
Liquid
Target:
Nprl3
Application Notes:
Application Notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 55kDa band observed in Human Heart lysates (calculated MW of 63.6kDa according to NP_853547.1). Recommended concentration: 0.3-1µg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch
orb1248785 (0.3 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
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