Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Form:
Liquid
Target:
NGFR
Application Notes:
Application Notes: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 75kDa band observed in Rat Heart lysates (calculated MW of 45.2kDa according to Human NP_002498.1 and 45.4kDa according to Rat NP_036742.2. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Nykjaer et al, Curr Opin Neurobiol. 2005 Feb,15(1):49-57). Recommended concentration: 1-3µg/mL
orb1248786 (1 ug/ml) staining of Rat Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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