Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Form:
Liquid
Target:
ULK3
Application Notes:
Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in Human Brain (Cerebellum, Cerebral Cortex, Hippocampus) lysates (calculated MW of 53.4kDa according to NP_001092906.1). Recommended concentration: 1-3µg/mL. An additional band of unknown identity was also consistently observed at 75kDa. This band was successfully blocked by incubation with the immunizing peptide.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371
orb1248808 (1 ug/ml) staining of Human Hippocampus lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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