Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Form:
Liquid
Target:
MTHFR
Application Notes:
Application Notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 70kDa band observed in lysates of cell line NIH3T3 (calculated MW of 74.6kDa according to Human NP_005948.3 and Mouse NP_034970.2). Recommended concentration: 0.3-1µg/mL
orb1248814 (0.3 ug/ml) staining of NIH3T3 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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