Western Blot Validation in Human Cell Lines and Mouse and Rat Tissues. Loading: 25 µg of lysates per lane. Antibodies: TMPRSS2 antibody, orb1255080, at 1:1000 dilution. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry Validation of TMPRSS2 in Human Esophageal Tissue. Immunohistochemistry of paraffin-embedded human esophageal tissue using TMPRSS2 antibody, orb1255080, at 1:100 dilution (40x lens).
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