Western Blot Validation in Human Cells and Mouse Kidney Tissue. Loading: 25 µg of lysates per lane. Antibodies: ACE2 antibody, orb1255081, at 1:3000 dilution. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST.
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