Western blot analysis of extracts of various cell lines, using ADAR antibody (orb1256130) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 20s.
Immunoprecipitation analysis of 200 ug extracts of HeLa cells using 3 ug SUZ12 antibody (orb1274421). Western blot was performed from the immunoprecipitate using SUZ12 antibody (orb1274421) at a dilution of 1:500.
Immunofluorescence analysis of HeLa cells using ADAR antibody (orb1256130) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded rat testis using ADAR antibody (orb1256130) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using ADAR antibody (orb1256130) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using ADAR antibody (orb1256130) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using ADAR antibody (orb1256130) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using ADAR antibody (orb1256130) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using ADAR antibody (orb1256130) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using ADAR antibody (orb1256130) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ADAR antibody (orb1256130) at dilution of 1:100 (40x lens).
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