Western blot analysis of extracts of various cell lines, using ASPSCR1 antibody (orb1256361) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat kidney using ASPSCR1 antibody (orb1256361) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using ASPSCR1 antibody (orb1256361) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using ASPSCR1 antibody (orb1256361) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using ASPSCR1 antibody (orb1256361) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using ASPSCR1 antibody (orb1256361) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using ASPSCR1 antibody (orb1256361) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using ASPSCR1 antibody (orb1256361) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of A-549 cells, using 3 ug ASPSCR1 antibody (orb1256361). Western blot was performed from the immunoprecipitate using ASPSCR1 antibody (orb1256361) at a dilution of 1:1000.
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