Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K18 antibody (orb1256501) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using LC3B antibody (orb1274430) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K18 antibody (orb1256501) at dilution of 1:100. NIH/3T3 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.
Dot-blot analysis of all sorts of methylation peptides using Acetyl-Histone H3-K18 antibody (orb1256501) at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat ovary using H3K18ac antibody (orb1256501) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using H3K18ac antibody (orb1256501) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using H3K18ac antibody (orb1256501) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using H3K18ac antibody (orb1256501) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using H3K18ac antibody (orb1256501) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using H3K18ac antibody (orb1256501) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using Acetyl-Histone H3-K18 antibody (orb1256501) at dilution of 1:100. C6 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.
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