H3K18ac Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1256501
Article Name: H3K18ac Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1256501
Supplier Catalog Number: orb1256501
Alternative Catalog Number: BYT-ORB1256501-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: ChIP, IF, IHC, IP, WB
Species Reactivity: Mouse, Rat
Immunogen: A synthetic acetylated peptide corresponding to residues surrounding K18 of human H3
Conjugation: Unconjugated
Alternative Names: H3/b, H3FB, ERBB, ERBB1, HER1, H3/a, H3/c, H3/d, H3/f, H3/h
H3K18ac Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 16kDa
UniProt: P68431
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: H3K18ac
Application Notes: Application Notes: WB: 1:1000 - 1:3000IHC: 1:200 - 1:500IF: 1:500 - 1:1000IP: 1:200 - 1:500CHIP: 1:50 - 1:100CHIP seq: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K18 antibody (orb1256501) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using LC3B antibody (orb1274430) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K18 antibody (orb1256501) at dilution of 1:100. NIH/3T3 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.
Dot-blot analysis of all sorts of methylation peptides using Acetyl-Histone H3-K18 antibody (orb1256501) at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat ovary using H3K18ac antibody (orb1256501) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using H3K18ac antibody (orb1256501) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using H3K18ac antibody (orb1256501) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using H3K18ac antibody (orb1256501) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using H3K18ac antibody (orb1256501) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using H3K18ac antibody (orb1256501) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using Acetyl-Histone H3-K18 antibody (orb1256501) at dilution of 1:100. C6 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.