H3K27me3 Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
BYT-ORB1258185
- Images (9)
| Article Name: | H3K27me3 Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | BYT-ORB1258185 |
| Supplier Catalog Number: | orb1258185 |
| Alternative Catalog Number: | BYT-ORB1258185-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ChIP, IF, IHC, IP, WB |
| Species Reactivity: | Human, Mouse, Other, Rat |
| Immunogen: | A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3 |
| Conjugation: | Unconjugated |
| Alternative Names: | H3F3A, H3t, H3.4, H3/g, H3FT |
| H3K27me3 Antibody |
| Clonality: | Polyclonal |
| Concentration: | batch dependent |
| Molecular Weight: | Observed: 17kDa |
| UniProt: | Q16695 |
| Buffer: | Liquid |
| Form: | Liquid |
| Target: | H3K27me3 |
| Application Notes: | Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100 |
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Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K27 antibody (orb1258185) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. |
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Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody (orb1258185). |
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Immunohistochemistry of paraffin-embedded rat ovary using TriMethyl-Histone H3-K27 antibody (orb1258185) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded human breast using TriMethyl-Histone H3-K27 antibody (orb1258185) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded mouse kidney using TriMethyl-Histone H3-K27 antibody (orb1258185) at dilution of 1:100 (40x lens). |
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Immunofluorescence analysis of 293T cells using TriMethyl-Histone H3-K27 antibody (orb1258185). Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of HeLa cells using TriMethyl-Histone H3-K27 antibody (Red) (orb1258185) and BrdU antibody (Green) (A1482). Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of HeLa cells using TriMethyl-Histone H3-K27 antibody (orb1258185) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of C6 cells using TriMethyl-Histone H3-K27 antibody (orb1258185) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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