COL1A1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1258836
Article Name: COL1A1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1258836
Supplier Catalog Number: orb1258836
Alternative Catalog Number: BYT-ORB1258836-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: A synthetic peptide of human COL1A1.
Conjugation: Unconjugated
Alternative Names: Alpha-1 type I collagenOI4
COL1A1 Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 139kDa
UniProt: P02452
Buffer: Liquid
Form: Liquid
Target: COL1A1
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using COL1A1 antibody (orb1258836) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry of paraffin-embedded human liver cancer using COL1A1 antibody (orb1258836) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using COL1A1 antibody (orb1258836) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using COL1A1 antibody (orb1258836) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using COL1A1 antibody (orb1258836) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using COL1A1 antibody (orb1258836) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded Rat heart using COL1A1 antibody (orb1258836) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using COL1A1 antibody (orb1258836) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of mouse kidney using COL1A1 antibody (orb1258836) at dilution of 1:100. Blue: DAPI for nuclear staining.