HSF1 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: HSF1 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: BYT-ORB1260257
• Supplier Catalog Number: orb1260257
• Alternative Catalog Number: BYT-ORB1260257-100
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 350-529 of human HSF1 (NP_005517.1).
• Conjugation: Unconjugated
• Alternative Names: HSF1, HSTF1

Product Description

HSF1 Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 90kDa
• UniProt: Q00613
• Buffer: Liquid
• Form: Liquid
• Target: HSF1

Application Notes

• Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100

Images

Western blot analysis of extracts of various cell lines, using HSF1 antibody (orb1260257) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat liver using HSF1 antibody (orb1260257) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using HSF1 antibody (orb1260257) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat spleen using HSF1 antibody (orb1260257) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human placenta using HSF1 antibody (orb1260257) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using HSF1 antibody (orb1260257) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using HSF1 antibody (orb1260257) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L929 cells using HSF1 antibody (orb1260257) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using HSF1 antibody (orb1260257) at dilution of 1:100. Blue: DAPI for nuclear staining.