Jun Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1261262
Article Name: Jun Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1261262
Supplier Catalog Number: orb1261262
Alternative Catalog Number: BYT-ORB1261262-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human c-Jun (NP_002219.1).
Conjugation: Unconjugated
Alternative Names: JUN, AP1, AP-1, c-Jun
Jun Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 43kDa
UniProt: P05412
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: Jun
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using c-Jun antibody (orb1261262) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunofluorescence analysis of NIH/3T3 cells using c-Jun antibody (orb1261262) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using c-Jun antibody (orb1261262) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded human colon using c-Jun antibody (orb1261262) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using c-Jun antibody (orb1261262) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using c-Jun antibody (orb1261262) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using c-Jun antibody (orb1261262) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using c-Jun antibody (orb1261262) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using c-Jun antibody (orb1261262) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using c-Jun antibody (orb1261262) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using Histone H2AX antibody (orb1255416) at dilution of 1:100. Blue: DAPI for nuclear staining.