Western blot analysis of extracts of various cell lines, using CTGF antibody (orb1261312) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat heart using CTGF antibody (orb1261312) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using CTGF antibody (orb1261312) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CTGF antibody (orb1261312) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using CTGF antibody (orb1261312) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using CTGF antibody (orb1261312) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using CTGF antibody (orb1261312) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using CTGF antibody (orb1261312) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using CTGF antibody (orb1261312) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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