Application Notes: ELISA:Sandwich:To detect hNP-1 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25 - 1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human NP-1 (XP-5247) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNP-1.Western Blot:To detect hNP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNP-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions
To detect hNP-1 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 - 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Human NP-1 (orb1272352) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNP-1.
To detect hNP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNP-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hNP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNP-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
* VAT and and shipping costs not included. Errors and price changes excepted
