Application Notes: ELISA:Sandwich:To detect hIL-1alpha by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25 - 1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human IL-1alpha (XP-5174) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-1alpha.Western Blot:To detect Rat IL-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions
To detect hIL-1-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 - 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Human IL-1-alpha (orb1272475) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-1-alpha.
To detect hIL-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hIL-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
* VAT and and shipping costs not included. Errors and price changes excepted
