Rabbit Anti-double stranded DNA Antibody IgM (dsDNA) ELISA Kit
Biozol Catalog Number:
BYT-ORB1291291
Supplier Catalog Number:
orb1291291
Alternative Catalog Number:
BYT-ORB1291291-48, BYT-ORB1291291-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
Rabbit
Alternative Names:
dsDNA Ab IgM
Rabbit dsDNA (anti-double stranded DNA antibody IgM) ELISA KIT
Concentration:
100 ng/mL
Range:
1.57-100 ng/mL
Sensitivity:
0.6 ng/mL
Samples:
serum, plasma, urine and other biological fluids
Application Notes:
Application Notes: standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit dsDNA IgM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit dsDNA IgM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit dsDNA IgM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rabbit dsDNA IgM in the samples is then determined by comparing the OD of the samples to the standard curve
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