Pig Calcitonin Gene Related Peptide 2 (CGRP2) ELISA Kit

Catalog Number: BYT-ORB1291353
Article Name: Pig Calcitonin Gene Related Peptide 2 (CGRP2) ELISA Kit
Biozol Catalog Number: BYT-ORB1291353
Supplier Catalog Number: orb1291353
Alternative Catalog Number: BYT-ORB1291353-48, BYT-ORB1291353-96
Manufacturer: Biorbyt
Category: Kits/Assays
Application: ELISA
Species Reactivity: Porcine
Alternative Names: CALCB, CGRP2, CALC2, CGRP-II, Calcitonin Gene Related Peptide 2, Calcitonin 2, Calcitonin Related Polypeptide Beta
Pig CGRP (Calcitonin Gene Related Peptide) ELISA Kit
Concentration: 1000 pg/mL
Range: 15.63-1000 pg/mL
Sensitivity: 5.14 pg/mL
Samples: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Application Notes: Application Notes: standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig CGRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig CGRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig CGRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Pig CGRP in the samples is then determined by comparing the OD of the samples to the standard curve