Human Tripartite motif-containing Protein 72 (TRIM72) ELISA Kit
Biozol Catalog Number:
BYT-ORB1291379
Supplier Catalog Number:
orb1291379
Alternative Catalog Number:
BYT-ORB1291379-48, BYT-ORB1291379-96
Manufacturer:
Biorbyt
Category:
Kits/Assays
Application:
ELISA
Species Reactivity:
Human
Alternative Names:
LOC493829, Mg53, Mitsugumin-53, TRI72_HUMAN, trim72, Tripartite motif containing 72 , Tripartite motif containing protein 72, Tripartite motif-containing protein 72
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TRIM72. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TRIM72. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TRIM72, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human TRIM72 in the samples is then determined by comparing the OD of the samples to the standard curve.
Application Notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TRIM72. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TRIM72. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TRIM72, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human TRIM72 in the samples is then determined by comparing the OD of the samples to the standard curve
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