Claudin-1/CLDN1 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Claudin-1/CLDN1 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: BYT-ORB1294235
• Supplier Catalog Number: orb1294235
• Alternative Catalog Number: BYT-ORB1294235-100,BYT-ORB1294235-25
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide
• Conjugation: Unconjugated

Product Description

Rabbit polyclonal antibody to Claudin-1

Product Properties

• Clonality: Polyclonal
• Target: Claudin-1 / CLDN1

Application Notes

• Application Dilute: Western Blot 1:1000 - 1:2000 Immunofluorescence 1:300 - 1:500

Images

Application: Immunocytochemistry (ICC), Sample: Mixture cell, Antibody: Claudin 1 antibody (orb1294235).

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294235 anti-Claudin 1 antibody (red) at 1:400 and 4C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Claudin-1 with orb1294235 at 1/100.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Claudin-1 with orb1294235 at 1/100.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Claudin-1 with orb1294235 at 1/100.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Claudin-1 with orb1294235 at 1/100.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Claudin-1 with orb1294235 at 1/100.

Immunohistochemical analysis of paraffin embedded Human prostate cancer tissue labeling Claudin-1 with orb1294235 at 1/100.

Lysates at 30 µg per lane. This blot was produced using a 15% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294235 overnight at 4C. anti-Claudin 1 antibody at 1/1000 dilution.