Synthetic peptide / Extracellular domain corresponding to Human CD138 / Syndecan-1
Conjugation:
Unconjugated
Rabbit polyclonal antibody to Syndecan-1
Clonality:
Polyclonal
Target:
Syndecan-1 / CD138 Extracellular domain
Application Dilute:
Western Blot 1:500-1:1000 Immunofluorescence 1:200-1:400 Immunohistochemistry (Paraffin) 1:200 - 1:300
All lanes: anti-Syndecan-1 / CD138 antibody at 1/1000 dilution, Lysates/proteins at 40 µg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294301 overnight at 4C.
All lanes: anti-Syndecan-1 / CD138 antibody at 1/1000 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294301 overnight at 4C.
All lanes: anti-Syndecan-1 / CD138 antibody at 1/500 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 5&10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294301 overnight at 4C.
All lanes: anti-Syndecan-1 / CD138 antibody at 1/500 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 5&10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294301 overnight at 4C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294301 anti-CD138 / Syndecan-1 antibody (red) at 1:200 and 4C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD138 / Syndecan-1 with orb1294301 at 1/200.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD138 / Syndecan-1 with orb1294301 at 1/200.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD138 / Syndecan-1 with orb1294301 at 1/200.
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