Synthetic peptide / Extracellular domain corresponding to Human CD44
Conjugation:
Unconjugated
Rabbit polyclonal antibody to CD44
Clonality:
Polyclonal
Target:
CD44
Application Dilute:
Western Blot 1:1000-1:2000 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1: 100-1:200
Anti-CD44 antibody - at 1/1000 dilution, Lysates at 40 µg per lane, This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294318 overnight at 4C.
Anti-CD44 antibody - at 1/1000 dilution, Lysates at 40 µg per lane, This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294318 overnight at 4C. Vimentin antibody (orb1294389).
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294318 anti-CD44 antibody (red) at 1:200 and 4C. DAPI (blue) was used as the nuclear counter stain.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294318 anti-CD44 antibody (red) at 1:200 and 4C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD44 with orb1294318 at 1/150.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD44 with orb1294318 at 1/150.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD44 with orb1294318 at 1/150.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD44 with orb1294318 at 1/150.
* VAT and and shipping costs not included. Errors and price changes excepted