NFE2L2 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: NFE2L2 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB1294327
• Supplier Catalog Number: orb1294327
• Alternative Catalog Number: BYT-ORB1294327-25,BYT-ORB1294327-100
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide / encompassing a sequence within the center region
• Conjugation: Unconjugated

Product Description

Rabbit polyclonal antibody to NRF2

Product Properties

• Clonality: Polyclonal
• Pubmed: 38508421
• Buffer: 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Target: NFE2L2 / NRF2

Application Notes

• Application Dilute: Western Blot 1:500-1:1000, Immunofluorescence 1:100-1:200, Immunohistochemistry (Paraffin) 1:100-1:200

Images

Anti-NRF2 antibody at 1/800 dilution. Lysates at 30 µg per lane. This blot was produced using a 5% SDS-PAGE. NC membrane was then blocked for an hour before being incubated with orb1294327 overnight at 4C.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling NRF2 with orb1294327 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling NRF2 with orb1294327 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling NRF2 with orb1294327 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling NRF2 with orb1294327 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling NRF2 with orb1294327 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294327 anti-NRF2 antibody (red) at 1:200 and 4C. DAPI (blue) was used as the nuclear counter stain.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294327 anti-NRF2 antibody (red) at 1:200 and 4C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling NRF2 with orb1294327 at 1/150.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling NRF2 with orb1294327 at 1/150.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling NRF2 with orb1294327 at 1/150.

Immunohistochemical analysis of paraffin embedded mouse Kidney tissue labeling NRF2 with orb1294327 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse liver labeling NRF2 with orb1294327 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse lung labeling NRF2 with orb1294327 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.