Synthetic peptide / encompassing a sequence within the C-terminus region.
Conjugation:
Unconjugated
Rabbit polyclonal antibody to TGF beta 1
Clonality:
Polyclonal
Target:
TGF beta 1
Application Dilute:
Western Blot 1:500 Immunofluorescence 1:100-1:200 Immunohistochemistry (Paraffin) 1:50-1:100
Anti TGF beta 1 antibody at 1/500 dilution. Lysates at 60 µg per lane. This blot was produced using a 12% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294344 overnight at 4C.
Anti TGF beta 1 antibody at 1/500 dilution. Lysates at 60 µg per lane. This blot was produced using a 12% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294344 overnight at 4C.
Anti TGF beta 1 antibody at 1/500 dilution. Lysates at 60 µg per lane. This blot was produced using a 12% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294344 overnight at 4C.
Cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294344 anti-TGF beta 1 antibody (red) at 1:200 and 4C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human Breast cancer tissue labeling TGF beta 1 with orb1294344 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling TGF beta 1 with orb1294344 at 1/100.
Immunohistochemical analysis of paraffin embedded Human Colorectal cancer tissue labeling TGF beta 1 with orb1294344 at 1/100.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling TGF beta 1 with orb1294344 at 1/100.
* VAT and and shipping costs not included. Errors and price changes excepted
