VEGF Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: VEGF Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB1294346
• Supplier Catalog Number: orb1294346
• Alternative Catalog Number: BYT-ORB1294346-25,BYT-ORB1294346-100
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide / encompassing a sequence within the center region
• Conjugation: Unconjugated

Product Description

Rabbit polyclonal antibody to VEGF

Product Properties

• Clonality: Polyclonal
• Buffer: 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Target: VEGF

Application Notes

• Application Dilute: Western Blot 1:500-1:1000, Immunofluorescence 1:200-1:500, Immunohistochemistry (Paraffin) 1:100-1:300, Immunoprecipitation : Reported in the literature, not verified in house.

Images

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294346 anti-VEGF antibody (red) at 1:200 and 4C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling VEGF with orb1294346 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling VEGF with orb1294346 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling VEGF with orb1294346 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

VEGF antibody at 1/1000 dilution. Lysates/proteins at 35 µg per lane. This blot was produced using a 15% SDS-PAGE.

VEGF antibody at 1/1000 dilution. Lysates/proteins at 35 µg per lane. This blot was produced using a 15% SDS-PAGE.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling VEGF with orb1294346 at 1/100.

Immunohistochemical analysis of paraffin embedded Human Colorectal cancer tissue labeling VEGF with orb1294346 at 1/100.

Immunohistochemical analysis of paraffin embedded Human Kidney cancer tissue labeling VEGF with orb1294346 at 1/100.

Immunohistochemical analysis of paraffin embedded Human Liver and Testis cancer tissue labeling VEGF with orb1294346 at 1/100.

Immunohistochemical analysis of paraffin embedded mouse colon labeling VEGF with orb1294346 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse Lung labeling VEGF with orb1294346 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling VEGF with orb1294346 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling VEGF with orb1294346 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.