Synthetic peptide / encompassing a sequence within the N-terminus region.
Conjugation:
Unconjugated
Rabbit polyclonal antibody to SQSTM1
Clonality:
Polyclonal
Buffer:
100mM Tris Glycine, 1% rAlbumin, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Target:
SQSTM1 / P62
Application Dilute:
Western Blot 1:1000-1:2000, Immunofluorescence 1:100-1:400, Immunohistochemistry (Paraffin) 1: 100-1:200
Anti-SQSTM1 / p62 antibody - at 1/1000 dilution. Lysates at 35 µg per lane. This blot was produced using a 12% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294360 overnight at 4C.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling SQS-TM1 / p62 with orb1294360 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling SQS-TM1 / p62 with orb1294360 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294360 anti-SQSTM1 / p62 antibody (red) at 1:200 and 4C. DAPI (blue) was used as the nuclear counter stain.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294360 anti-SQSTM1 / p62 antibody (red) at 1:100 and 4C. DAPI (blue) was used as the nuclear counter stain.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294360 anti-SQSTM1 / p62 antibody (red) at 1:100 and 4C. DAPI (blue) was used as the nuclear counter stain.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294360 anti-SQSTM1 / p62 antibody (red) at 1:100 and 4C. DAPI (blue) was used as the nuclear counter stain.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294360 anti-SQSTM1 / p62 antibody (red) at 1:100 and 4C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human Colorectal cancer tissue labeling SQSTM1 / p62 with orb1294360 at 1/200.
Immunohistochemical analysis of paraffin embedded mouse kidney labeling SQS / TM1 / P62 with orb1294360 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded mouse spleen labeling SQS / TM1 / P62 with orb1294360 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
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