Synthetic peptide / Extracellular domain corresponding to Human VCAM-1 / CD106
Conjugation:
Unconjugated
Rabbit polyclonal antibody to VCAM-1
Clonality:
Polyclonal
Target:
VCAM-1 / CD106 (Extracellular)
Application Dilute:
Western Blot 1:500-1:1000 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1:100-1:200
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294364 anti-VCAM-1 antibody (red) at 1:200 and 4C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling VCAM-1 / CD106 with orb1294364 at 1/100.
Immunohistochemical analysis of paraffin embedded Human colorectal cancer tissue labeling VCAM-1 / CD106 with orb1294364 at 1/100.
Immunohistochemical analysis of paraffin embedded Human placenta tissue labeling VCAM-1 / CD106 with orb1294364 at 1/100.
VCAM-1 / CD106 antibody - at 1/1000 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294364 overnight at 4C.
VCAM-1 / CD106 antibody - at 1/1000 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294364 overnight at 4C.
VCAM-1 / CD106 antibody - at 1/1000 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294364 overnight at 4C.
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