CD45 (Extracellular) Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1294367
Article Name: CD45 (Extracellular) Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1294367
Supplier Catalog Number: orb1294367
Alternative Catalog Number: BYT-ORB1294367-100,BYT-ORB1294367-25
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: FC, IF, IHC, WB
Species Reactivity: Human, Mouse
Immunogen: Synthetic peptide / Extracellular domain corresponding to Human CD45
Conjugation: Unconjugated
Rabbit polyclonal antibody to CD45 antibody (Extracellular domain)
Clonality: Polyclonal
Target: CD45 (Extracellular)
Application Dilute: Western Blot 1:500-1:1000 Immunofluorescence 1:200-1:400 Immunohistochemistry (Paraffin) 1:400-1:800
All lanes: Anti-CD45 antibody at 1/1000 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294367 overnight at 4C.
All lanes: Anti-CD45 antibody at 1/1000 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294367 overnight at 4C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294367 anti-CD45 antibody (red) at 1:200 and 4C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human Breast cancer tissue labeling CD45 with orb1294367 at 1/400.
Immunohistochemical analysis of paraffin embedded Human Colorecta cancer tissue labeling CD45 with orb1294367 at 1/400.
Immunohistochemical analysis of paraffin embedded Human Liver cancer tissue labeling CD45 with orb1294367 at 1/400.
Immunohistochemical analysis of paraffin embedded Human Lung cancer tissue labeling CD45 with orb1294367 at 1/400.
CD45 antibody, orb1294367, Cell: THP-1.