alpha-Smooth Muscle Actin Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB1294387
- Images (11)
| Article Name: | alpha-Smooth Muscle Actin Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB1294387 |
| Supplier Catalog Number: | orb1294387 |
| Alternative Catalog Number: | BYT-ORB1294387-25,BYT-ORB1294387-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Synthetic peptide / encompassing a sequence within the center region |
| Conjugation: | Unconjugated |
| Rabbit polyclonal antibody to alpha Smooth Muscle Actin |
| Clonality: | Polyclonal |
| Buffer: | 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
| Target: | alpha-Smooth Muscle Actin |
| Application Dilute: | Western Blot 1:1000-1:3000, Immunofluorescence 1:200-1:500, Immunohistochemistry (Paraffin) 1:100-1:400, Immunohistochemistry (Frozen) 1:100-1:200 |
|
|
Anti-alpha Smooth Muscle Actin antibody at 1/2000 dilution, Lysates at 35 µg per lane, This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294387 overnight at 4C. |
|
|
Immunohistochemical analysis of paraffin embedded mouse tissue labeling alpha Smooth Muscle Actin with orb1294387 at 1/300. |
|
|
Immunohistochemical analysis of paraffin embedded mouse tissue labeling alpha Smooth Muscle Actin with orb1294387 at 1/300. |
|
|
Application: Immunocytochemistry (ICC), Sample: Mixture cell, Antibody: alpha Smooth Muscle Actin antibody (orb1294387). |
|
|
Frozen sectioned for immunohistochemical analysis. Sample: Frozen section. Red: alpha Smooth Muscle Actin antibody. Catalog: orb1294387 at 1-200. |
|
|
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294387 anti-alpha Smooth Muscle Actin antibody (red) at 1:400 and 4C. DAPI (blue) was used as the nuclear counter stain. |
|
|
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294387 anti-alpha Smooth Muscle Actin antibody (red) at 1:400 and 4C. DAPI (blue) was used as the nuclear counter stain. |
|
|
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling alpha Smooth Muscle Actin with orb1294387 at 1/200. |
|
|
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling alpha Smooth Muscle Actin with orb1294387 at 1/200. |
|
|
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling alpha Smooth Muscle Actin with orb1294387 at 1/200. |
|
|
Immunohistochemical analysis of paraffin embedded mouse tissue labeling alpha Smooth Muscle Actin with orb1294387 at 1/300. |
Product Guarantee and Expert Support
